Figure 1. Overview of BAC recombineering in E. coli.

BSMs were amplified by PCR using tailed oligonucleotide primers: 1) Primer tail sequences were designed to introduce 80 bp of homology to the target locus, at both of the 5′ and 3′ extremities of the BSM. 2) Replacement of target locus with BSM. 3) Heat-induction of homologous recombination functions mediate by lambda phage in E. coli.