| AmpRF |
GGTCTGACAGTTACCAATGC
|
Plasmid construction |
| Hyg-R |
GCTTGATATCGAATTCGTCG
|
Plasmid construction |
| Zeo1F |
GAATTCTCAGTCCTGCTCCT
|
Plasmid construction |
| Zeo1R |
CGGGGGATCCACTAGTTCT
|
Plasmid construction |
| PtrAF |
GGCCAATTGATTACGGGAT
|
Plasmid construction |
| PtrAR |
ATGGCCTCTTGCATCTTTG
|
Plasmid construction |
| pacC BSM-A/H -F |
GCGAGCGTCACGTCGGTCG
AAAGAGCACAAATAACCTT
AACCTGACATGCCAGTGGG
GAAGCTGCCGCACCACGACT
GTTGGTCTGACAGTTACCAATGC
|
5′-flanking amplificationand fusion |
| BSM-A/H -R |
TGCTCCTTCAATATCAGTTA
ACGTCGACGAGGAAATGTG
CGCGGAACCCCTATTTGTTTA
|
5′-flanking amplification |
| pacC BSM-A/H -R |
TGGATGGAGGGGCGACGCTC
TTCGGGGCGAGCACGCTGTAA
AGTGCCTCCAGTGTACCGGCG
ACGATCATCGTCAAAGATGCTTG
ATATCGAATTCGTCG
|
3′-flanking amplification and fusion |
| BSM-A/H -F |
TAAACAAATAGGGGTTCCGCGCAC
ATTTCCTCGTCGACGTTAACTG
ATATTGAAGGAGCA
|
3′-flanking amplification |
| pacCzeo-F |
GCGAGCGTCACGTCGGTCGAA
AGAGCACAAATAACCTTAACCT
GACATGCCAGTGGGGAAGCTGC
CGCACCACGACTGTTTTCTAGAG
CGGCCGCGATAT
|
BSM-Z/P biselectable marker amplification
|
| pacCPtrA-R |
TGGATGGAGGGGCGACGCTCT
TCGGGGCGAGCACGCTGTAAA
GTGCCTCCAGTGTACCGGCGA
CGATCATCGTCAAAGATGGCC
TAGATGGCCTCTTGCA
|
BSM-Z/P biselectable markeramplification |
| CF5R |
ATAAGGTTAGCCGAGATGCG
|
pacC replacement verification |
| CF5F |
CTAGCACTTCCATGAGCAAC
|
pacC replacement verification |
| 520A-F |
CAAAGCCACATCGACCCTT
GCCCTCTGGCCGGATCACACCT
GGACAAGCTACCCTCCTCTATC
GCGTCCTCTTCTTCCTGGGTCT
GACAGTTACCAATGC
|
BSM-A/H biselectable markeramplification |
| 520H-R |
GCCGCATCCATATCCAAGCG
TGATCTGTAGCTATGTCCCAC
TAGGTCTACTCAATGGCATTG
TCAGGTCCAGTCCGCCTTGCT
TGATATCGAATTCGTCG
|
BSM-A/H biselectable markeramplification |
| 520-1F |
GCCCTCTGGCCGGATCACAC
|
AFUA_8G00520 deletionverification |
| 520-1R |
CATGGGGACTGGCCGCATCC
|
AFUA_8G00520 deletionverification |
| 550A-F |
CGGTCATAGACAAGAGGAATCT
CTACATAAAGGCGCTATCCCTGC
TATTAAATGACGGGCATGGGATT
GGTAGTTCGTAGGGTCTGACA
GTTACCAATGC
|
BSM-A/H biselectable markeramplification |
| 550H-R |
CGATTGTACATGCTCACACGTA
GAATCGGCACAGTCTTGGGAA
AAGTATGTGGTTTAACTAGCC
TTGTCGACCTTGCTTTGCTTGA
TATCGAATTCGTCG
|
BSM-A/H biselectable marker amplification |
| 550-1F |
CGGGCATGGGATTGGTAGTTCGT
|
AFUA_8G00550 deletionverification |
| 550-1R |
GGCCTAACCGGGTTCCAGCG
|
AFUA_8G00550 deletionverification |
| PsoACA-F |
AAGGCGGACTGGACCTGACA
ATGCCATTGAGTAGACCTAGT
GGGACATAGCTACAGATCAC
GCTTGGATATGGATGCGGCG
GTCTGACAGTTACCAATGC
|
BSM-A/H biselectable markeramplification |
| PsoACH-R |
TCAGCACTAGGGAAGTCGGT
GTAATGGTGTCAGCCTACTCA
GTCACGTGCAGGACATAATCC
TCCATCCCCCGAACGACAGCTT
GATATCGAATTCGTCG
|
BSM-A/H biselectable markeramplification |
| PSOAClustF1 |
CAGCCTGTGGCTCGCTGGTC
|
PsoA cluster deletionverification |
| PSOAClustR1 |
TCCCCGCGTCCACACTCGAT
|
PsoA cluster deletionverification |
| 520SB-F |
TTCAGGTGCTGCAAGATGTC
|
Southern Blot probe |
| 520SB-R |
CTTCATGGCCGTTCTGGTAT
|
Southern Blot probe |
| 550SB-F |
GGCCTGATCTACCTTCACCA
|
Southern Blot probe |
| 550SB-R |
TAGCAGGGATAGCGCCTTTA
|
Southern Blot probe |
| 640_F |
CCCCTTGACATAGGGTAATAAT
GTGCTTTCGCATTGTTCCACCC
ATGGCCCCCCCGCGTTCGGAG
CTGCGTTAGCTAGGCTTCT
AGAGCGGCCGCGATAT
|
BSM-Z/P biselectable markeramplification |
| 640_R |
TTATTTGCTGGCATCTCGCAA
CTTCTCAAGAAGATGGACCAA
GTTATCCACCAGTGGCGGAGT
CTGTTTAGAAGTTTCATATGGCC
TCTTGCATCTTTG
|
BSM-Z/P biselectable markeramplification |
| 640_INT_F |
ATTCGGCTCTGCATATCACC
|
regA disruption verification |
| 640_INT_R |
TGAATGATAGGCGTCCTTCC
|
regA disruption verification |
| 5′_640 |
TCCAGGATCTTCGCATAGGT
|
regA disruption verification |
| 3′_640 |
GACCGAGTTGACTCGGATCT
|
regA disruption verification |
