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| Type | Compound | Retention time(min) | Transitions | Conevoltage | Collisionenergy |
| Target | Pseurotin A | 3.95 | 432>316 | 30 | 10 |
| 432>348 | 15 | 10 | |||
| Internal Standard (1) | 3-F Pseurotin A | 3.59 | 450>334 | 15 | 5 |
| 450>366 | 15 | 5 | |||
| Internal Standard (2) | 2-F Pseurotin A | 4.63 | 450>344 | 15 | 5 |
| 450>366 | 15 | 5 |
*In bold is the quantification transition.
