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. 2014 Nov 5;9(11):e112247. doi: 10.1371/journal.pone.0112247

Figure 2. POU4F3-mediated activation of PRE1, PRE2 and a 4.2 kb Nr2f2 5′ flanking sequence.

Figure 2

a. Schematic diagram of reporter constructs used in luciferase reporter assays in ND7 cells. POU4F3 recognition element (PRE) 1 and 2 are shown in an Nr2f2 5′ flanking region fragment (grey box) cloned upstream of a luciferase reporter gene (LUC). The location of this fragment relative to the Nr2f2 transcriptional start site is shown. b. Evaluation of the response of 4.2 kb-Nr2f2-Luc to increasing levels of POU4F3 or dreidel mutant POU4F3 (Ddl) expression construct. The luciferase activity of the reporter is normalised to its response to the empty expression vector and results are expressed relative to this. c. Response of the PRE1-Luc reporter construct in co-transfection experiments with POU4F3 and dreidel expression constructs. d. Evaluation of the response of PRE2-Luc in similar experiments to c. Error bars represent the s.e.m in b, c and d (n = 6 for each data point).