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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Mol Cancer Ther. 2014 Sep 9;13(11):2515–2526. doi: 10.1158/1535-7163.MCT-14-0319

Figure 3. BTC and TTC-352 inhibit T47D:A18/PKCα and T47D:A18-TAM1 colony formation in 3D Matrigel™ and induce apoptosis in MCF-7:5C cells in vitro.

Figure 3

Colonies were established as described in Materials and Methods and treated for 10 days (Control [0.1% DMSO], E2 1 nM, BTC 100 nM, TTC-352 100 nM). A) T47D:A18/neo. B) T47D:A18/PKCα. C) T47D:A18-TAM1. Proliferation assay was performed following 9 days of treatment. D) MCF-7:WS8. E) MCF-7:5C. F) Apoptosis was assessed in MCF-7:5C cells following 6 days of treatment as described in Materials and Methods. *, P ≤ 0.05 versus DMSO. **, P ≤ 0.01 versus DMSO. ***, P ≤ 0.001 versus DMSO. Graph shows mean ± SEM of 3 independent experiments.