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. Author manuscript; available in PMC: 2015 Oct 16.
Published in final edited form as: Immunity. 2014 Oct 9;41(4):592–604. doi: 10.1016/j.immuni.2014.09.009

Figure 2. Human B Cells Express Functional LOX-1.

Figure 2

(A) LOX-1 expression on peripheral B cell subsets.

(B) Purified total B cells were cultured overnight in the presence or absence of 10 μg/ml αIgM, 100 ng/ml αCD40 or 50 nM CpG ODN2006. LOX-1 expression was assessed.

(C) Purified and CFSE-labeled total B cells were cultured in plates coated with αLOX-1 or control IgG for 7 days. Cells were stained with CD19 and CD38.

(D) On day 12 of the B cell culture in (C), culture supernatants were analyzed for measuring Igs by ELISA.

(E) Purified total B cells were cultured overnight in plates coated with αLOX-1 or control IgG. B cells were stained for CD40, CCR6, CXCR5 and CCR7.

(F) B cells in (E) were added to the upper wells of transwells containing the indicated concentrations of CCL27 or CCL19 in the lower wells. After 2 hours, the number of cells that migrated into the lower wells was determined using counting beads. Two independent experiments using cells from two different donors showed similar results. In D and F, error bars indicate mean±SD of triplicate assays in individual experiments.