Table 1.

Sequences of primers used for qPCR

Genes	Description	Primers
CYCB	cyclophillin B	L 5′tgtggtgtttggcaaagttc-3′ R 5′-gtttatcccggctgtctgtc-3′
β-actin	β-actin	L 5′-ccaaccgcgagaagatga-3′ R 5′-ccagaggcgtacagggatag-3′
ALDOA	aldolase A, fructose-bisphosphate	L 5′- cgggaagaaggagaacctg-3′ R 5′-gaccgctcggagtgtacttt-3′
PDK1	pyruvate dehydrogenase kinase, isozyme 1	L 5′-ccgctctccatgaagcagtt-3′ R 5′-ttgccgcagaaacataaatgag-3′
HK2	hexokinase 2	L 5′-caaagtgacagtgggtgtgg-3′ R 5′-gccaggtccttcactgtctc-3′
MCT2	monocarboxylate transporter 2	L 5′-atcctgggcttcattgacat-3′ R 5′-atcctgggcttcattgacat-3′
MCT4	monocarboxylate transporter 4	L 5’atcctgggcttcattgacat-3’ R 5’-atggagaagctgaagaggta-3’
GLUT1	glucose transporter 1	L 5′-ccattggctccggtatcgt-3′ R 5′-tgctcgctccaccacaaac-3′
NHEI	sodium hydrogen exchanger	L 5′-tctgccgtctccactgtctcca-3′ R 5′-cccttcagctcctcattcacca-3′
LDHA	lactate dehydrogenase A	L 5′-acccagtttccaccatgatt-3′ L 5′-cccaaaatgcaaggaacact-3′
VEGFA	vascular endothelial growth factor A	L 5′-tggaattggattcgccattt-3′ R 5′-tgggtgggtgtgtctacagga-3′
TYR	tyrosinase	L 5′-ggctgttttgtactgcctgct-3′ R 5′-aggagacacaggctctagggaa-3′
Cyp11A1, exon 4	cytochrome P450, family 11, subfamily A, polypeptide 1, exon 4	L 5′ ccagacctgttccgtctgtt-3′ R 5′ aaaatcacgtcccatgcag-3′
Cyp11B1	cytochrome P450, family 11, subfamily B, polypeptide 1	L 5′-aggtggacagcctgcatc-3′ R 5′-ccattcaggcccattcag-3′
Cyp17A1	cytochrome P450, family 17, subfamily A, polypeptide 1	L 5′-gcatcatagacaacctgagcaa-3′ R 5′-gggttttgttggggaaaatc-3′
Cyp21A2v2	cytochrome P450, family 21, subfamily A, polypeptide 2,variant2	L 5′-gcttggcctgactcagaaat-3′ R 5′-gacaccagcttgtcttgcag-3′
HSD11B1	hydroxysteroid (11-beta) dehydrogenase 1	L 5′-caatggaagcattgttgtcg-3′ R 5′-ggcagcaaccattggataag-3′
HSD11B2	hydroxysteroid (11-beta) dehydrogenase 2	L 5′-gtcaaggtcagcatcatcca-3′ R 5′-cactgacccacgtttctcac-3′
7DHCR	7-dehydrocholesterol reductase	L 5′-ttgtaaaagaaattgcctgtgaat-3′ R 5′-gccatggtcaagggctac-3′
3βHSD	3β-hydroxysteroid dehydrogenase	L 5′-cttggacaaggccttcagac-3′ R 5′-tcaagtacagtcagcttggtcct-3′
CRH	corticotropin releasing hormone	L 5′-ctccgggaagtcttggaaat-3′ R 5′-gttgctgtgagcttgctgtg-3′
CRH, exon2	corticotropin releasing hormone, exon 2	L 5′-actcagagaccaagtc ca-3′ R cttcccaggcgcttcgcaggt-3′
CRHR1	corticotropin releasing hormone receptor 1	L 5′-tggatgttcatctgcattgg-3′ R 5′-tgccaaaccagcacttctc-3′
POMC, exon3	proopiomelanocortin, exon 3	L 5′-_agcctcagcctgcctggaa-3′ R 5′- cagcaggttgctttccgtggtg-3′
MC1	melanocortin receptor 1	L 5′-actccgtctgctccaatgac-3′ R 5′-agagctggcagcaaagatg-3′
MC2	melanocortin receptor 2	L 5′-tcttcagcctgtctgtgattg-3′ R 5′-ggcacaggatgaagaccag-5′
PCSK1	proprotein convertase subtilisin/kexin type 1	L 5′-tgatcccacaaacgagaaca-3′ R 5′-tgtgattatttgcttgcatgg-3′
PCSK2	proprotein convertase subtilisin/kexin type 2	L 5′-agcatacaactccaaggttgc-3′ R 5′-aggcctcgatgatgtctgtc-3′
NQO2	NAD(P)H dehydrogenase, quinone2	L 5′-ttgacatcccaggattctacg-3′ R 5′-ccgtggttacggaaaggag-3′
EGFR	epidermal growth factor receptor	L 5′-acacagaatctatacccaccagagt-3′ R 5′-atcaactcccaaacggtcac -3′
ICAM1	intercellular adhesion molecule 1	L 5′-ccttcctcaccgtgtactgg-3′ R 5′-agcgtagggtaaggttcttgc-3′
IFNα1	interferon α1	L 5′-ccctctctttatcaacaaacttgc-3′ L 5′-ttgttttcatgttggaccaga-3′
IFNβ1	interferon β1	L 5′-gatgagtacaaaagtcctgatcca-3′ R 5′-ctgcagccactggttctgt-3′
IL1α	interleukin 1α	L 5′-ggttgagtttaagccaatcca-3′ R 5′-tgctgacctaggcttgatga-3′
IL1β	interleukin 1β	L 5′-crgtcctgcgtgttgaaaga-3′ R 5′-ttgggtaatttttgggatctaca-3′
IL2	interleukin 1	L 5′-aagttttacatgcccaagaagg-3′ R 5′-aagtgaaagtttttgctttgagcta-3′
IL4	interleukin 3	L 5′-caccgagttgaccgtaacag-3′ R 5′-gccctgcagaaggtttcc-3′
IL5	interleukin 5	L 5′-ggtttgttgcagccaaagat-3′ R 5′-tcttggccctcattctcact-3′
IL6 (IFNβ2)	interleukin 6	L 5′-gaagctctatctcgcctcca-3′ R 5′-agcaggcaacaccaggag-3′
IL8 (CXCL-8)	interleukin 8, chemokine ligand 8	L 5′-agacagcagagcacacaagc-3′ R 5′-atggttccttccggtggt-3′
IL10	interleukin 10	L 5′-tgggggagaacctgaagac-3′ R 5′-ccttgctcttgttttcacagg-3′
IL17A	interleukin 17A	L 5′-tgggaagacctcattggtgt-3′ R 5′-ggatttcgtgggattgtgat-3′
TNFα	tumor necrosis factor alpha	L 5′-cagcctcttctccttcctgat-3′ R 5′-gccagagggctgattagaga-3′
IFNγ	interferon gamma	L 5′-ggcattttgaagaattggaaag-3′ R 5′-tttggatgctctggtcatctt-3′
PDGFA	platelet-derived growth factor alpha polypeptide	L 5′-gcagtcagatccacagcatc-3′ R 5′-tccaaagaatcctcactcccta-3′
TGFA	transforming growth factor, alpha	L 5′-ttgctgccactcagaaacag-3′ R 5′-atctgccacagtccacctg-3′
TGFB1	transforming growth factor, beta 1	L 5′-gcagcacgtggagctgta-3′ R 5′-cagccggttgctgaggta-3′
TGFB2	transforming growth factor, beta 2	L 5′-ccaaagggtacaatgccaac-3′ R 5′-cagatgcttctggatttatggtatt-3′
RANTES	CCL5 chemokine (C-C motif) ligand 5	L 5′-tgcccacatcaaggagtattt-3′ R 5′-tttcgggtgacaaagacga-3′
TRANSFERRIN RC	transferrin receptor	L 5′-ttgagaaaacaatgcaaaatgtg-3′ R 5′-cccagttgctgtcctgatataga-3′
NPL	N-acetylneuraminate pyruvate lyase	L 5′-ccagagatttatcaactttgttgtca-3′ R 5′-gaggctttctgcagtggaag-3′