Table 5.
Melanogenesis stimulates expression of several genes regulated by HIF-1.
| Mean Cp value ± S.D. | ||
|---|---|---|
| Gene | Non-pigmented cells | Pigmented cells |
| VEGF | 6.04 ±0.31 | 0.85±0.18 |
| GLUT1 | 6.55 ±0.28 | 0.11±0.31 |
| Tyr | 4.68 ±0.22 | 3.05±0.10 |
| LDHA | 9.34 ±0.16 | 3.05±0.10 |
| MCT2 | 3.52 ±0.17 | 0.16±0.15 |
| NHEI | 9.34 ±0.16 | −3.09±0.26 |
| PDK1 | −2.12 ±0.33 | −8.25±0.16 |
| ALDOA | 0.04 ±0.35 | −5.11±0.31 |
| HK2 | 0.38 ±0.16 | −4.77±0.32 |
Expression of mRNAs in non-pigmented versus pigmented SKMEL-188 melanoma cells grown as described as in the methods was compared to β-actin using a comparative CP method. Relative gene expression data were calculated using the ΔΔCp method. Changes in gene expression are presented as a relative quantities using the mean ΔCp (normalized target) ± S.D. to caculate the difference between the target gene and the reference gene by comparing the first cycle of appearance above threshold (crossing point, Cp) using standardized algorithms in the Roche LC480 1.5 software package. All data (pigmented vs non-pigmented cells) are statistically significant, p <0.0001. VEGFA (vascular endothelial growth factor A); GLUT-1 (glucose receptor 1); Tyr (Tyrosinase); LDHA (lactate dehydrogenase A); MCT2 (monocarboxylate transporter 2); NHEI (sodium hydrogen exchanger); PDK1 (pyruvate dehydrogenase kinase, isozyme 1); ALDOA (aldolase A, fructose-bisphosphate); HK2 (hexokinase 2). A lower Cp numeric value reflects a more abundant signal for each particular gene (PCR product) of interest.