Table 7.
Pigmentation-dependent gene expression in human SKMEL-188 melanoma cells.
| Mean Cp ± S.D. | ||
|---|---|---|
| Gene | Non Pigmented Cells | Pigmented Cells |
| CRH | 4.51±0.37 | 3.14±0.63 * |
| CRH, exon 2 | 5.24±0.28 | 3.14±0.63 ** |
| CRHR1 | 3.45±0.36 | 1.53±0.72 * |
| POMC, exon 3 | 5.24±0.28 | 2.02±0.66 ** |
| MC1 | 5.24±0.28 | −3.01±0.69 **** |
| MC2 | 3.07±0.31 | 2.02±0.67 |
| PCSK1 | 5.24±0.28 | 3.14±0.63 ** |
| PCSK2 | 5.24±0.28 | −31.86±0.001 **** |
| NQO2 | 5.24±0.28 | −2.89±0.65 **** |
| Cyp11A1, exon 4 | 5.24±0.26 | 3.14±0.63 ** |
| Cyp11B1 | 4.16±0.31 | 0.17±0.64 *** |
| 3βHSD | 5.24±0.28 | 3.14±0.63 ** |
| Cyp17A1 | −10.70±0.3 | 0.15±0.63 **** |
| Cyp21A2v2 | −29.76±0.001 | 3.14±0.063 **** |
| HSD11B1 | −12.86±0.42 | −4.38±0.64 **** |
| HSD11B2 | −13.48±0.45 | −4.04±0.7 **** |
| 7DHCR | −1.87±0.34 | 3.14±0.63 *** |
| ICAM1 | 11.41±0.19 | 11.78±0.25 |
| IFNα1 | −0.07±0.20 | 0.25±0.26 |
| IFNβ1 | 7.41±0.31 | 7.83±0.26 |
| IFNγ | −23.59±0.1 | −23.22±0.1 |
| TNFα | 7.02±0.35 | 7.12±0.32 |
| IL1α | 11.41±0.19 | 11.78±0.25 |
| IL1β | 11.38±0.20 | 11.41±0.41 |
| IL2 | 11.41±0.19 | 11.78±0.25 |
| IL4 | 9.47±0.22 | 9.77±0.81 |
| IL5 | −6.13±0.36 | 11.14±0.41 **** |
| IL6 | 7.28±0.21 | 7.86±0.32 |
| IL8 (CXCL-8) | 11.41±0.19 | 11.78±0.25 |
| IL10 | 10.57±0.41 | 11.56±0.46 * |
| IL17A | −4.89±0.47 | 7.78±0.25 **** |
| RANTES | 11.41±0.19 | 11.78±0.25 |
| PDGFA | −0.61±0.21 | 6.84±0.30 **** |
| TGFA | 3.59±0.34 | 4.29±0.40 |
| TGFB1 | 9.33±0.24 | 9.82±0.27 |
| TGFB2 | 0.37±0.20 | −23.22±0.001 **** |
| EGFR | 7.10±0.34 | 7.57±0.28 |
| TRANSFERRIN RC | −11.69±0.37 | −5.61±0.42 **** |
| NPL | 11.41±0.19 | 11.78±0.25 |
Changes in gene expression of human SKMEL-188 melanoma cells grown for three days in culture in F10 media (non-pigmented) or in DMEM media (pigmented) as described in the methods, after normalization to the reference gene cyclophillin B. Changes in gene expression are presented as a relative quantities using mean ΔCp (normalized target) ± S.D. as the difference between target gene and reference gene in cycle of appearance in time (Cp). Data were compared between pigmented and non-pigmented cells using Student's t-test. Genes profiled include: CYP11A1 (cytochrome P450, family 11, subfamily A, polypeptide 1) exon 4; CYP11B1 (cytochrome P450, family 11, subfamily B, polypeptide 1); CYP17A1 (cytochrome P450, family 17, subfamily A, polypeptide 1); CYP21 A2v2 (cytochrome P450, family 21, subfamily A, polypeptide 2); HSD11B1 (hydroxysteroid (11-beta) dehydrogenase 1); HSD11B1 (hydroxysteroid (11-beta) dehydrogenase 2); 7DHCR (7-dehydrocholesterol reductase); 3βHSD (3-beta-hydroxysteroid dehydrogenase); CRH (corticotropin releasing hormone); CRHR1 (corticotropin releasing hormone receptor 1); POMC (proopiomelanocortin); MC (melanocortin receptor); PCSK (proprotein convertase subtilisin/kexin type); NQO2 (NAD(P)H dehydrogenase, quinone 2); EGFR (epidermal growth factor receptor); ICAM1 (intercellular adhesion molecule 1); IFN (interferon); IL (interleukin); CXCL (chemokine ligand); TNF (tumor necrosis factor); PDGFA (platelet-derived growth factor alpha polypeptide); TGFB (transforming growth factor, beta); RANTES (CCL5 chemokine (C-C motif) ligand 5); TRANSFERRIN RC (transferrin receptor); NPL (N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase)
p <0.05
p <0.01
p <0.001
p <0.0001.
A lower Cp numeric value reflects a more abundant signal for each particular gene (PCR product) of interest.