a. Western blot analysis comparing phosphorylated Erk1/2, Erk1/2 and GAPDH expression in 451 Lu cells transfected or treated with the following; Lane 1 miRZip scrambled hairpin RNA control, Lane 2 miR-340 inhibiting miRZip340, Lane 3 PCMV-MIR over expression control, Lane 4 PCMV-MIR340 miR-340 over expression, Lane 5 DMSO control, Lane 6 50mM MEK1 inhibitor PD98059. b. GAPDH normalized densitometry of phosphorylated Erk1/2 expression amongst treatment groups. Presented as relative expression of phosphorylated Erk1/2. c. Expression of 43 components in the MAPK pathway upon inhibition of miR-340 in 451 Lu melanoma cells. 48 hours after transfection cells were collected and assayed for mRNA expression by qRT-PCR, normalized to TATA-binding protein and presented as fold-change in transcript expression ± SD, relative to scrambled hairpin RNA control. Changes in transcript expression are significant (p ≤ 0.01), except where p value is noted in figure and not considered significant. Insert. miR-340 expression levels 48 hours after transfection in 451 Lu cells transfected with miRZip scrambled hairpin RNA control (left) or miR-340 inhibiting miRZip340 (right). Normalized to RNU6B and presented as percentages of control.