Table 1.
Experimental design (CCD) of response surface methodology
| Std runs | (A) temperature (°C) | (B) pH | (C) yeast extract (g L -1) | (D) incubation period (days) | % of dye degradation | Laccase production (UmL -1) | ||
|---|---|---|---|---|---|---|---|---|
| Experimental | Predicted | Experimental | Predicted | |||||
| 1.0 | 10.0 | 3.0 | 5.0 | 0.0 | 0.20 | 0.82 | 0.02 | 0.22 |
| 2.0 | 40.0 | 3.0 | 5.0 | 0.0 | 1.50 | 2.61 | 0.03 | 0.11 |
| 3.0 | 10.0 | 11.0 | 5.0 | 0.0 | 1.60 | 2.23 | 0.02 | 0.14 |
| 4.0 | 40.0 | 11.0 | 5.0 | 0.0 | 1.20 | 4.43 | 0.01 | 0.22 |
| 5.0 | 10.0 | 3.0 | 9.0 | 0.0 | 0.20 | 0.44 | 0.20 | 0.15 |
| 6.0 | 40.0 | 3.0 | 9.0 | 0.0 | 1.20 | 2.77 | 0.32 | 0.05 |
| 7.0 | 10.0 | 11.0 | 9.0 | 0.0 | 1.20 | 2.14 | 0.01 | 0.05 |
| 8.0 | 40.0 | 11.0 | 9.0 | 0.0 | 3.50 | 4.87 | 0.02 | 0.66 |
| 9.0 | 10.0 | 3.0 | 5.0 | 15.0 | 87.71 | 93.26 | 2.71 | 2.85 |
| 10.0 | 40.0 | 3.0 | 5.0 | 15.0 | 87.01 | 94.76 | 2.01 | 2.23 |
| 11.0 | 10.0 | 11.0 | 5.0 | 15.0 | 87.74 | 94.87 | 2.74 | 2.97 |
| 12.0 | 40.0 | 11.0 | 5.0 | 15.0 | 86.80 | 96.78 | 1.80 | 2.76 |
| 13.0 | 10.0 | 3.0 | 9.0 | 15.0 | 87.15 | 92.61 | 2.15 | 2.20 |
| 14.0 | 40.0 | 3.0 | 9.0 | 15.0 | 86.45 | 94.64 | 1.45 | 2.11 |
| 15.0 | 10.0 | 11.0 | 9.0 | 15.0 | 86.70 | 94.50 | 1.70 | 2.61 |
| 16.0 | 40.0 | 11.0 | 9.0 | 15.0 | 87.87 | 96.94 | 2.87 | 2.92 |
| 17.0 | 5.0 | 7.0 | 7.0 | 7.5 | 88.39 | 78.25 | 3.39 | 2.92 |
| 18.0 | 55.0 | 7.0 | 7.0 | 7.5 | 88.45 | 72.64 | 3.45 | 2.58 |
| 19.0 | 25.0 | 1.0 | 7.0 | 7.5 | 86.54 | 76.42 | 1.54 | 1.53 |
| 20.0 | 25.0 | 15.0 | 7.0 | 7.5 | 87.17 | 72.55 | 2.17 | 1.10 |
| 21.0 | 25.0 | 7.0 | 3.0 | 7.5 | 88.99 | 78.89 | 3.99 | 3.51 |
| 22.0 | 25.0 | 7.0 | 11.0 | 7.5 | 88.89 | 78.67 | 3.89 | 3.29 |
| 23.0 | 25.0 | 7.0 | 7.0 | 7.5 | 87.55 | 86.98 | 2.55 | 3.19 |
| 24.0 | 25.0 | 7.0 | 7.0 | 22.5 | 87.24 | 66.93 | 2.24 | 1.16 |
| 25.0 | 25.0 | 7.0 | 7.0 | 7.5 | 88.90 | 86.98 | 3.90 | 3.19 |
| 26.0 | 25.0 | 7.0 | 7.0 | 7.5 | 88.90 | 86.98 | 3.90 | 3.19 |
| 27.0 | 25.0 | 7.0 | 7.0 | 7.5 | 88.90 | 86.98 | 3.90 | 3.19 |
| 28.0 | 25.0 | 7.0 | 7.0 | 7.5 | 88.90 | 86.98 | 3.90 | 3.19 |
| 29.0 | 25.0 | 7.0 | 7.0 | 7.5 | 88.90 | 86.98 | 3.90 | 3.19 |
| 30.0 | 25.0 | 7.0 | 7.0 | 7.5 | 86.74 | 86.98 | 1.74 | 3.19 |