Table 3.
Analysis of variance table (ANOVA) for response surface methodology of main effects and interacting effects of parameters in quadratic model for the laccase production by Hypocrea lixii
| Source | Sum of squares | df | Mean | F value | p-value Prob > F |
|---|---|---|---|---|---|
| Model | 48.56277 | 14 | 3.468769 | 2.621997 | 0.0371* |
| X1-Temperature (°C) | 0.000179 | 1 | 0.000179 | 0.000136 | 0.0009*** |
| X2-pH | 0.717105 | 1 | 0.717105 | 0.54205 | 0.00729** |
| X3-Yeast extract (g L-1) | 0.069738 | 1 | 0.069738 | 0.052714 | 0.8215NS |
| X4-Incubation period (days) | 25.37625 | 1 | 25.37625 | 19.18158 | 0.0005*** |
| X1X2 | 0.16657 | 1 | 0.16657 | 0.125908 | 0.7277NS |
| X1X3 | 0.278689 | 1 | 0.278689 | 0.210657 | 0.6528NS |
| X1X4 | 0.085404 | 1 | 0.085404 | 0.064556 | 0.8029 NS |
| X2X3 | 0.081467 | 1 | 0.081467 | 0.06158 | 0.8074 NS |
| X2X4 | 0.040612 | 1 | 0.040612 | 0.030698 | 0.8633 NS |
| X3X4 | 0.075826 | 1 | 0.075826 | 0.057316 | 0.8140 NS |
| X1 2 | 0.395835 | 1 | 0.395835 | 0.299206 | 0.5924 NS |
| X2 2 | 7.268732 | 1 | 7.268732 | 5.494339 | 0.0333* |
| X3 2 | 0.080502 | 1 | 0.080502 | 0.06085 | 0.8085 NS |
| X4 2 | 19.98253 | 1 | 19.98253 | 15.10453 | 0.0015** |
| Residual | 19.84424 | 15 | 1.322949 | ||
| Lack of Fit | 6.769842 | 9 | 0.752205 | 0.345196 | 0.9261NS |
| Pure Error | 13.0744 | 6 | 2.179066 | ||
| Cor Total | 68.40701 | 29 |
Statistically significant ***(P <0.0001), **(P < 0.01), *(P < 0.05), NS Non-significant.