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. Author manuscript; available in PMC: 2015 Oct 2.
Published in final edited form as: Oncogene. 2014 May 5;34(14):1811–1821. doi: 10.1038/onc.2014.116

Figure 5.

Figure 5

Talin1 S425 phosphorylation promotes bone colonization in vivo. (a) Luciferase-labeled PC3-MM2 sh-control, talin1-silenced, talin1WT and mutant-expressing cells (1 × 106) were injected into nude mice intracardially. After 21 days injection, bioluminescence and X-ray imaging of mice were superimposed to localize tumor growth. Arrow indicates tumor growth in femur/tibia junction. *Compared to talin1WT, P < 0.05. (b) Tumor burden in the femur/tibia of mice following intracardiac injection. Tumor growth was estimated by luciferase activity (presented as photons/sec). (c-d) PC3-MM2 talin1WT and mutant-expressing cells (5 × 105) were orthotopically injected into the prostate. (c) Sample tumor sizes of lymph node metastases. (d) Bioluminescence imaging of lymph node metastases of PC3-MM2 talin1WT and mutant-expressing cells in mice after removing the primary tumor.