| Collagen mix components | Volume of each (3 ml) | Calculate the total volume needed for the number of rafts desired |
| 8 parts collagen type 1 | 2.4 ml | —— ml |
| 1 part Reconstitution buffer | 0.3 ml | —— ml |
| 1 part 10× DMEM | 0.3 ml | —— ml |
| J2 3T3 fibroblasts | 5 × 105 cells | —— × 106 cells |
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