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. 2014 Nov 5;9(11):e112220. doi: 10.1371/journal.pone.0112220

Figure 3. Effects of NPRL-Z-1 on cell cycle distribution and expression of cell cycle-related proteins in A498 cells.

Figure 3

Cells were incubated with (A) DMSO or various concentrations of NPRL-Z-1 for 24 h and (B) DMSO or 10 µM NPRL-Z-1 for the indicated time periods. Cell cycle phase and cell apoptosis were determined by FACS as described in Materials and Methods. (C) A498 cells were incubated with DMSO or 10 µM NPRL-Z-1 for the indicated time periods. After treatment, cells were harvested and lysed for detection of the indicated proteins via western blotting. Data are expressed as the mean percentage of control ± S.D. of three independent experiments. *, # p<0.05, and ## p<0.01 compared with the control group.