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. 2014 Nov 5;9(11):e112220. doi: 10.1371/journal.pone.0112220

Figure 4. Effects of NPRL-Z-1 on DNA DSBs and DNA checkpoint pathway.

Figure 4

(A) A498 cells were seeded and treated with NPRL-Z-1 or etoposide for 30 min and processed for the comet assay as detailed in Materials and Methods. (B) A498 cells were incubated with DMSO or 3 or 10 µM NPRL-Z-1 for the indicated time periods. After treatment, cells were harvested and lysed for detection of the expression of indicated protein via western blotting. N3, N10 and E25 indicated as NPRL-Z-1 3 µM, 10 µM and etoposide 25 µM, respectively.