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. 2014 Nov 5;9(11):e112220. doi: 10.1371/journal.pone.0112220

Figure 7. Effects of TOP2 in NPRL-Z-1-induced cell death and DNA damage signaling.

Figure 7

TOP2α or TOP2β siRNA was transfected to evaluate expression of TOP2α (A) and TOP2β (B), cell viability using the MTT assay (C and D), expression of apoptosis-related proteins (PARP and pro-caspase-3) (E and F) for 24 h, and DNA damage-related proteins (p-ATM, p53, and p-histone H2AX) (G and H) for 1 h in A498 cells. Data are expressed as the mean percentage of control ± S.D. of three independent experiments. *, & p<0.05, and **, ##, && p<0.01 compared with the treatment group. N3, N10 and E25 indicated as NPRL-Z-1 3 µM, 10 µM and etoposide 25 µM, respectively.