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. 2014 Nov 5;9(11):e112220. doi: 10.1371/journal.pone.0112220

Figure 8. Effect of NPRL-Z-1 on cellular ROS accumulation in A498 cells.

Figure 8

A498 cells were incubated in the absence or presence of NPRL-Z-1 (10 µM) for indicated time. The fluorescent intensity of DCFH-DA was detected by flow cytometric analysis. ** P<0.01 compared with the 0.5 h-time point control group. ## P<0.05 compared with the 1 h-time point control group. && P<0.01 compared with the 3 hr-time point control group. (B) Different ROS scavengers were preincubated for 30 min and cell viability was determined by MTT assay. *** P<0.001 compared with the control group. # P<0.05 compared with the NPRL-Z-1-treated group. Treatment of NAC inhibited NPRL-Z-1-induced ROS generation (C, ** P<0.01 and ## P<0.01 compared with the NPRL-Z-1-treated group.). (D) NAC (10 mM) reduced the apoptosis-related results in NPRL-Z-1-treated cells for 24 h. E25 indicated as etoposide 25 µM.