Skip to main content
. 2014 Nov 5;3:16–24. doi: 10.1016/j.redox.2014.10.001

Fig. 6.

Fig. 6

Effect of cholesterol on AA activation of membrane fraction and trimera. (A) AA activation of membrane fraction and trimera (1) In the control experiment, membrane fraction and the trimera were incubated together during 4 min in the presence of 40 µM AA. The rate obtained corresponds to the 100% value (75 mol O2•−/s/mol Cyt b558). (2–4) Membrane fractions (MF) and trimera were separately preincubated during 10 s and mixed together for 4 min as follow: (2) both were preincubated with 40 µM AA, (3) only MF was preincubated with 40 µM AA, (4) only the trimera were preinicubated with 40 µM AA. (5) The rate was measured in absence of trimera. (6) The rate was measured in absence of MF. (B) Membrane fractions (MF) and trimera were separately preincubated during 10 s and mixed together for 4 min as follow: ■ only MF was preincubated with 40 µM AA plus cholesterol as indicated, ▲ only the trimera was preincubated with 40 µM AA plus cholesterol as indicated and ● both were preincubated with 40 µM AA plus cholesterol. Activities in Fig. 6B were expressed as the percent of activities measured of Fig. 6A corresponding to each state to assess the cholesterol effect only.