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Mechanism of deglutathionylation and detection of Pr–SSG. a Schematic representation of the method to detect glutathionylated proteins by labeling with Dylight 488 maleimide. b Deglutathionylation assay is divided by three steps. (1) The Pr–SSG is reduced by Grx. (2) The Grx-SSG intermediate is transferred to GSH. (3) Glutathione reductase (GR) reduces GSSG with presence of NAPDH
