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. Author manuscript; available in PMC: 2014 Nov 6.
Published in final edited form as: Anal Bioanal Chem. 2011 Aug 13;401(7):2165–2175. doi: 10.1007/s00216-011-5311-x

Fig. 2.

Fig. 2

In vitro S-glutathionylated BSA (BSA-SSG) and validation of the method using controls. 1 Electropherograms of Dylight 488 maleimide; 2 BSA-SSG; 3 BSA-SSG without Grx reduction; 4 BSA-SSG pretreated with DTT; 5 native BSA. Hydrodynamic injection at pH 8 and 11 kPa for 4 s; separation in gel containing 15% dextran (64–76 kDa), 0.5% SDS, 20 mM Tris, and 20 mM Tricine at −570 V/cm. 1, 2 Indicate Dylight 488 maleimide. Top trace is offset in the Y axis for the clarity