Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Oct 31;9:4. doi: 10.1186/1747-1028-9-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 Uzunova et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Independent nuclear localization of the subunits of the Tof1/Csm3/Mrc1 complex. (A) Viability test of the S-phase checkpoint GFP-tagged proteins by 10-fold serial dilution assay. 5 μL of each dilution are spotted onto YPD, YPD supplemented with 100 mM and 200 mM HU. (B-D) All GFP strains are paraformaldehyde fixed and subjected to fluorescent microscopy analysis to detect the position of GFP-tagged proteins in the cell. 2.5 μg/ml DAPI staining is used for all of the probes to visualize the position of nucleus. The obtained GFP and DAPI signals are analyzed for co-localizations. GFP - Filter set 38HE (Zeiss); DAPI - Filter set 01 (Zeiss); BF – bright field.