Fig. 5.

Effect of signal transduction inhibitors on the PACAP38-induced decrease in viability of Y79 cells. Following 1 h pretreatment with U0126 (MEK1/2 inhibitor; 10 μM); SB203580 (SB, p38 inhibitor; 10 μM); SP600125 (SP, JNK inhibitor; 10 μM) cells were incubated for 24 h with PACAP38 (2 μM). Cell viability was analyzed by MTT test. Data are presented as mean ± SEM of 6–12 values per group and expressed as a percentage of the respective control (C). a indicates p < 0.001 vs. control, b indicates p < 0.001 vs. PACAP38