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. 2014 Oct 10;46(10):e117. doi: 10.1038/emm.2014.61

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Copyright © 2014 KSBMB.

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/

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Suppression of growth hormone receptor (GHR) alters key signaling molecules in PANC-1 and HPAC cells. (a, c, e) The effect of GHR knockdown on AKT/phosphatidylinositol 3 kinase (PI3K) signaling was analyzed in pancreatic cancer cells. The expression of phospho-AKT, AKT, phospho-PI3K, PI3K, phospho-phosphatase and tensin homolog (PTEN), phospho-mammalian target of rapamycin (mTOR), mTOR, phospho-p70s6kinase, p70s6kinase and the internal control β-actin was measured by western blotting. (b, d, f) Densitometric analysis is also shown to the right of each representative image. Data presented as the mean±s.d. (n=3). *P<0.05.