Additional file 1: Movie 1.

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3D monoculture of MDA-MB-231 cells (green), showing that cells in the aggregate dissociate and become spindle-shaped. Figure 1C shows snapshots of the top-half area of this movie. Many spindle-shaped cells become round and vice versa in the bottom-left area. The large mass in the upper half of the field shows active dissociation of cells. A mass of cells on the right side of the field appearing in the second half of the film shows movement of round cells within the mass. The images were captured every minute at a single Z-plane. Duration of filming was 14 hours and 53 minutes.