Table 1.
Primers and conditions for PCR detection of pathogen DNA
| Organism | Target gene | Primer | Sequence (5' to 3') | Amplicon | Annealing temperature (°C) | Reference |
|---|---|---|---|---|---|---|
| size (bp) | ||||||
| Rickettsia spp | gltA | RpCS.780p | GACCATGAGCAGAATGCTTCT | 600 | 48 | [8] |
| RpCS.877p | GGGGACCTGCTCACGGCGG | 480 | 54 | |||
| RpCS.1273r | CATAACCAGTGTAAAGCTG | |||||
| Anaplasma sp.. | 16S rDNA | EHR16SD | GGTACCYACAGAAGAAGTCC | 345 | 53 | [9] |
| EHR16SR | TAGCACTCATCGTTTACAGC | |||||
| Coxiella burnetii | IS1111 | Trans 1 | TATGTATCCACCGTAGCCAGTC | 687 | 60 | [10] |
| Trans 2 | CCCAACAACACCTCCTTATTC | |||||
| Borrelia spp. | fla gene | BflaPAD | GATCA(G/A)GC(T/A)CAA(C/T)ATAACCA(A/T)ATGCA | 55 | [11] | |
| BflaPDU | AGATTCAAGTCTGTTTTGGAAAGC | |||||
| BflaPBU,nest | GCTGAAGAGCTTGGAATGCAACC | 340 | 55 | |||
| BflaPCR,nest | TGATCAGTTATCATTCTAATAGCA | |||||
| B. microti | 18S rDNA | Babl | CTTAGTATAAGCTTTTATACAGC | 238 | 55 | [12] |
| Bab4 | ATAGGTCAGAAACTTGAATGATACA | |||||
| Trypanosoma spp. | ITS1 rDNA | ITS1 CF | CCGGAAGTTCACCGATATTG | Variable | 58 | [13] |
| ITS1 BR | TTGCTGCGTTCTTCAACGAA | |||||
| Leishmania spp. | kDNA minicircle | L.MC-1S | CTRGGGGTTGGTGTAAAATAG- | 700 | 55 | [14] |
| L.MC-1R | TWTGAACGGGRTTTCTG | |||||
| Plasmodium spp. | Cytb | DW2 &DW4 | DW2; TAATGCCTAGACGTATTCCTGATTATCCAG | 1253 | 60 | [15] |
| DW4; TGTTTGCTTGGGAGCTGTAATCATAATGTG | ||||||
| Cytb1 & Cytb2 | CYTb1; CTCTATTAATTTAGTTAAAGCACA | 939 | 50 | |||
| Tb2; ACAGAATAATCTCTAGCACC |