Table 3.
In vitro kinetics of the cumulative gas production with the pea flours (first gas test trial)
Cumulative gas production (mL 200 mg−1 DM) | P-value | ||||||||
---|---|---|---|---|---|---|---|---|---|
Incubation time (h) | PCF | GPCF | PFF | GPFF | SEM | F | G | F × G | Run |
1 | 2.6a | 4.8b | 4.6b | 4.9b | 0.15 | <0.001 | <0.001 | <0.001 | 0.017 |
2 | 5.6a | 9.2b | 9.1b | 9.4b | 0.27 | <0.001 | <0.001 | <0.001 | <0.001 |
4 | 10.6a | 18.5b | 17.9b | 18.5b | 0.38 | <0.001 | <0.001 | <0.001 | <0.001 |
6 | 15.6a | 33.2b | 32.1b | 33.0b | 0.61 | <0.001 | <0.001 | <0.001 | <0.001 |
8 | 20.9a | 48.0b | 49.1b | 49.7b | 0.93 | <0.001 | <0.001 | <0.001 | 0.007 |
12 | 35.4a | 60.3b | 63.4b | 62.9b | 1.51 | <0.001 | <0.001 | <0.001 | 0.73 |
24 | 75.2a | 71.3a | 74.8a | 74.1a | 1.42 | 0.40 | 0.13 | 0.25 | 0.23 |
32 | 82.2a | 75.8b | 79.0a,b | 78.9a,b | 1.48 | 0.97 | 0.04 | 0.05 | 0.12 |
48 | 87.7a | 79.5b | 83.2b | 82.8b | 1.52 | 0.69 | 0.01 | 0.02 | 0.04 |
Kinetic fitting parameters | RSE | ||||||||
---|---|---|---|---|---|---|---|---|---|
a | 89.2a | 75.6b | 79.3b | 78.8b | 1.69 | 0.17 | 0.03 | 0.03 | |
c | 0.123a | 0.244b | 0.243b | 0.244b | 0.0635 | <0.001 | <0.001 | <0.001 | |
l | 2 h 43 mina | 1 h 7 minb | 1 h 20 minb | 1 h 13 minb | 26 min | 0.11 | 0.05 | 0.07 | |
GP16 (mL 200 mg−1 DM) | 54.7a | 71.1b | 74.3b | 74.1b | 0.99 | <0.001 | <0.001 | <0.001 |
Data are presented as LSMEANS ± SEM. PCF, pea coarse flour, obtained with a crushing roller with a 2.5 mm space; GPCF, PCF reground with a screen of 1 mm aperture; PFF, pea fine flour, obtained with hammer mill with a 2 mm screen; GPFF, PFF reground with a screen of 1 mm aperture; F, feed effect (coarse vs. fine); G, regrinding effect; F × G, interaction between feed and regrinding effects; RSE, residual standard error of the model; GP16, gas production after 16 h and 40 min. Mean values in the same row without a common superscript are significantly different at P < 0.05.