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. 2014 Apr 4;2(4):308–320. doi: 10.1002/fsn3.90

Table 3.

In vitro kinetics of the cumulative gas production with the pea flours (first gas test trial)

Cumulative gas production (mL 200 mg−1 DM) P-value


Incubation time (h) PCF GPCF PFF GPFF SEM F G F × G Run
1 2.6a 4.8b 4.6b 4.9b 0.15 <0.001 <0.001 <0.001 0.017
2 5.6a 9.2b 9.1b 9.4b 0.27 <0.001 <0.001 <0.001 <0.001
4 10.6a 18.5b 17.9b 18.5b 0.38 <0.001 <0.001 <0.001 <0.001
6 15.6a 33.2b 32.1b 33.0b 0.61 <0.001 <0.001 <0.001 <0.001
8 20.9a 48.0b 49.1b 49.7b 0.93 <0.001 <0.001 <0.001 0.007
12 35.4a 60.3b 63.4b 62.9b 1.51 <0.001 <0.001 <0.001 0.73
24 75.2a 71.3a 74.8a 74.1a 1.42 0.40 0.13 0.25 0.23
32 82.2a 75.8b 79.0a,b 78.9a,b 1.48 0.97 0.04 0.05 0.12
48 87.7a 79.5b 83.2b 82.8b 1.52 0.69 0.01 0.02 0.04
Kinetic fitting parameters RSE
a 89.2a 75.6b 79.3b 78.8b 1.69 0.17 0.03 0.03
c 0.123a 0.244b 0.243b 0.244b 0.0635 <0.001 <0.001 <0.001
l 2 h 43 mina 1 h 7 minb 1 h 20 minb 1 h 13 minb 26 min 0.11 0.05 0.07
GP16 (mL 200 mg1 DM) 54.7a 71.1b 74.3b 74.1b 0.99 <0.001 <0.001 <0.001

Data are presented as LSMEANS ± SEM. PCF, pea coarse flour, obtained with a crushing roller with a 2.5 mm space; GPCF, PCF reground with a screen of 1 mm aperture; PFF, pea fine flour, obtained with hammer mill with a 2 mm screen; GPFF, PFF reground with a screen of 1 mm aperture; F, feed effect (coarse vs. fine); G, regrinding effect; F × G, interaction between feed and regrinding effects; RSE, residual standard error of the model; GP16, gas production after 16 h and 40 min. Mean values in the same row without a common superscript are significantly different at P < 0.05.