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Determination of mature miR-172 copy number. (A) Standard curves were constructed for mature miR-172 using synthetic miR-172 standard sample. Tenfold dilutions of miR-172 RNA oligos was quantified by real-time PCR. (B) The assays produced a 6-log dynamic range, lower limit of detection of 2 × 10 copies and an R2 > 0.99.
