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. 2014 May 15;2(4):380–388. doi: 10.1002/fsn3.113

Table 1.

Gene-specific primers used to amplify the miRNAs precursors and mature miRNA

Gene Forward primer (5′→3′) Reverse primer (5′→3′)
Pre-miR-172a GCAGCACCATCAAGATTCAC GCAGCATCATCAAGATTCTCATA
Pre-miR-172b CAGCACCATTAAGATTCACAT CAGCATCATCAAGATTCTCATA
RT-miR-172 CTCAACTGGTGTCGTGGAGTCGGCAATTCAGT TGAGATGCAGCA
miR-172 ACACTCCAGCTGGGAGAATCTTGATGATGC
RT-miR-824 CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTCCCTTCT
miR-824 ACACTCCAGCTGGGTAGACCATTTGTGAGA TGGTGTCGTGGAGTC