Figure 1.

(a) Schematic view of the breath sampling device. Left: the exploded view of the device assembly. Right: the assembled device (not to scale). The space between the glass tube and outer casing is filled with cooling material (dry ice pellets), and the tube is locked in place with end-caps. For breath collection, the soft mask on the bottom of the device is placed around a dolphin’s blowhole. When the animal inhales, the inhale one-way valve opens, while the exhale valve closes and air is routed from the intake; when the animal exhales, the inhale valve closes, the exhale valve opens, and exhaled air is expelled through the chilled glass tube out of the exhaust. (b) Sampling breath from a trained dolphin.