Table 1.
Sites identified to be under functional divergence between GAS6 and PROS1. Functional divergence analysis was performed using three different methods: FunDi (FD) [59], BADASP (B) [57] and Selectome (PS) [62,63]. BADASP is a package to detect both Type I and Type II of functional divergence. Type I sites are divided into Ia (amino acid conserved in PROS1 and divergent in GAS6) or Ib (amino acid conserved in GAS6 and divergent in PROS1). FunDi aims to detect sites under functional divergence, independent of whether they belong to Type I or Type II of functional divergence. For GAS6, numbering is based on isoform 1 or 2 (between brackets). Overall, the methionine encoded by the translation initiation site is numbered as residue 1.
| GAS6 | AA | PROS1 | AA | methods | GAS6 | AA | PROS1 | AA | methods |
|---|---|---|---|---|---|---|---|---|---|
| 37 (37) | E | 31 | Q | B_Ib | 384 (341) | Q | 345 | D | B_Ia |
| 51 (51) | Q | 44 | S | PS | 405 (362) | N | 366 | E | B_Ia, B_Ib |
| 60 (60) | H | 53 | N | B_II | 415 (372) | P | 376 | D | B_II, PS |
| 97 (97) | N | 90 | R | B_Ia, B_Ib | 423 (380) | Q | 384 | N | B_Ib |
| 98 (98) | K | 91 | S | B_Ia, B_II | 432 (389) | R | 393 | H | FD, B_Ib |
| 100 (100) | G | 93 | Q | B_II | 435 (392) | V | 396 | S | FD, B_II, PS |
| 102 (102) | P | 103 | S | B_Ia | 445 (402) | K | 406 | D | B_Ib |
| 105 (105) | K | 106 | A | B_Ib, B_II | 448 (405) | V | 409 | K | FD, B_Ib |
| 106 (106) | N | 107 | Y | B_Ib | 455 (412) | P | 416 | P | B_Ia |
| 110 (110) | A | 111 | R | FD, B_Ia, B_II | 456 (413) | E | 417 | E | B_Ib |
| 114 (114) | Q | 115 | N | B_Ia | 457 (414) | R | 418 | N | B_Ia |
| 123 (123) | N | 124 | L | B_Ia, B_Ib | 463 (420) | N | 424 | K | B_II |
| 134 (134) | Q | 135 | K | B_Ib | 465 (422) | T | 426 | Y | FD |
| 136 (136) | L | 137 | G | FD | 471 (428) | F | 432 | R | FD, B_Ia, B_Ib, B_II |
| 141 (141) | F | 142 | T | FD | 473 (430) | E | 434 | V | B_II |
| 143 (143) | L | 144 | T | B_Ia | 496 (453) | G | 458 | Q | FD, B_II |
| 146 (146) | A | 147 | P | B_Ia | 497 (454) | E | 459 | G | B_II |
| 161 (161) | S | 162 | K | B_Ib | 498 (455) | D | 460 | A | B_II |
| 162 (162) | Q | 163 | D | FD | 508 (465) | N | 470 | K | B_II |
| 170 (170) | I | 174 | I | B_Ib | 510 (467) | R | 472 | N | B_Ia, B_Ib, B_II |
| 189 (189) | S | 193 | L | B_Ia | 512 (469) | Q | 474 | H | B_Ib, B_II |
| 192 (192) | G | 196 | K | B_Ia | 517 (474) | T | 479 | V | FD |
| 203 (203) | D | 207 | L | B_II | 518 (475) | E | 480 | E | FD, B_Ia |
| 204 (204) | S | 209 | P | B_Ib | 526 (483) | S | 488 | S | B_Ia |
| 218 (218) | S | 223 | D | B_Ib | 585 (542) | Y | 542 | S | B_Ib, B_II |
| 222 (222) | L | 227 | E | B_II | 586 (543) | H | 543 | T | FD, B_Ia, B_Ib |
| 224 (224) | D | 229 | P | B_Ia, B_Ib | 588 (545) | T | 545 | E | B_Ia, B_Ib |
| 248 (248) | E | 253 | A | B_II | 593 (550) | K | 547 | S | B_Ib |
| 266 (266) | G | 271 | K | B_Ia, B_Ib, B_II | 595 (552) | L | 549 | D | B_Ia, B_Ib, B_II |
| 274 (274) | M | 279 | Q | FD | 614 (571) | D | 569 | S | B_Ib |
| 322 (279) | D | 284 | V | B_Ib | 618 (575) | H | 573 | S | FD, B_Ib |
| 332 (289) | A | 294 | D | B_Ia, B_Ib | 623 (580) | S | 578 | R | FD, B_Ib |
| 337 (294) | S | 299 | L | B_II | 626 (583) | D | 581 | R | B_Ia |
| 343 (300) | M | 305 | Q | PS | 639 (596) | Q | 594 | T | FD |
| 351 (308) | R | 312 | Y | B_II, PS | 640 (597) | S | 595 | I | B_Ib |
| 356 (313) | R | 317 | L | B_II | 641 (598) | E | 596 | S | FD, B_Ib |
| 357 (314) | L | 318 | P | B_Ia, B_Ib, PS | 657 (614) | H | 610 | A | B_Ia, B_II, PS |
| 381 (338) | G | 342 | E | B_II | 703 (660) | Y | 656 | S | FD, B_Ia, B_Ib |
| 383 (340) | H | 344 | I | FD, B_Ia, B_Ib | 717 (674) | E | 670 | W | B_Ib |