Effects of knockdown of FOXO on juvenile hormone signaling and lipid metabolism. A. Relative transcript levels of Met, gce, bmm, mdy, Kr-h1, E75, JHAMT, and FOXO following injection of siFOXO. B. Total lipid levels in female flies after siGFP, siFOXO, or siMet/siGCE treatments, respectively. Each point represents the mean ± SE of eight flies. C. Number of pupae deposited per female over 40d after siGFP, siFOXO, or siMet/siGCE treatments, respectively, mean ± SE of three groups of 30 flies. D. Duration of the first gonotrophic cycle after siGFP, siFOXO, or siMet/siGCE treatments, respectively, mean ± SE of three groups of 15 flies. Significant differences (indicated by “a” at P < 0.01) were determined by ANOVA followed by Tukey's test compared to controls. siGFP, short interfering green fluorescent protein.