TABLE 5 .
Primers used for real-time PCR analysis of genes in the inositol phosphate metabolism pathway that were found in the P. murina, P. carinii, and P. jirovecii genomes but not in the S. pombe genome
| Species and target | Primer |
Annealing temp (°C) | |
|---|---|---|---|
| Left | Right | ||
| P. murina | |||
| Inositol-polyphosphate multikinase (EC 2.7.1.151) | AGAGCCTGATTTTGACATCGA | AGCCTCTTCCCATATCTTTTGCA | 55 |
| Inositol-pentakisphosphate 2-kinase (EC 2.7.1.158) | GGCATATTTCTCAGAAGGAAACCG | ATGAAAGAGGGGAGTCACAACC | 55 |
| Phosphoinositide 5-phosphatase (EC 3.1.3.36) | TTGATAGGGCCAATGAATTTTC | TCTGACTTATATTTTCGCCCTGA | 55 |
| Inositol-1,4-bisphosphate 1-phosphatase (EC 3.1.3.57) | TTTGTCAAAGCCATGCACCG | ACCTGCAGCATGATCCCAAG | 56 |
| P. carinii | |||
| Inositol-polyphosphate multikinase (EC 2.7.1.151) | TGGCTATATAAAGGGTTTGCGA | AGCCTCTTCCCATATCTTTTGCA | 60 |
| Inositol-pentakisphosphate 2-kinase (EC 2.7.1.158) | AGTCGTAACTCCCCTCTTTCAT | CGCATGTGTTTCGTTAATGTCT | 55 |
| Phosphoinositide 5-phosphatase (EC 3.1.3.36) | AAAGGCTCTAATTACGTCAACT | TGATCAGAAAATGTCAATGGTGA | 50 |
| Inositol-1,4-bisphosphate 1-phosphatase (EC 3.1.3.57) | TGTTAGCACGTGGGGAATGT | ACCTGCTGCATGATCCCAAA | 60 |