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MetR increases production of isopentenol by 55%. The eight tolerance genes were individually coexpressed with the isopentenol production pathway. The tolerance genes and the production pathway were simultaneously induced with 500 µM IPTG in triplicate, and the isopentenol concentration was measured 24, 48, and 72 h after induction. The production strain coexpressing metR (PS+MetR) improved the isopentenol titer 55% over that for the rfp-expressing strain (PS+RFP).
