Figure 5.

N-acetyl cysteine exposure during Chlamdyia/HSV-2 co-infection rescues EB production during Chlamydia/HSV-2 co-infection. (A) Triplicate HeLa monolayers were either CtE- or CtE/HSV-2-infected. Monolayers were harvested at 30 min and 20 h pvi for quantification of GSH and GSSG. (B) Triplicate HeLa monolayers were either CtE- or CtE/HSV-2-infected. (C) Triplicate HeLa monolayers were either Cm- or Cm/HSV-2-infected. Following HSV-2 infection, cultures were replenished with either standard culture medium (Ctrl) or medium containing NAC (5 mM). Monolayers were harvested at 20 h pvi for EB titration analysis. Results are expressed as the mean ±s.e.m. of three biological replicates. Significant (P ≤ 0.05) difference between Chlamydia singly-infected and Chlamydia/HSV-2 co-infected samples is indicated by the asterisk (^*).