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. 2014 Nov 6;5:599. doi: 10.3389/fpls.2014.00599

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Copyright © 2014 Rocheta, Sobral, Magalhães, Amorim, Ribeiro, Pinheiro, Egas, Morais-Cecílio and Costa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Relative expression of differentially expressed male and female genes chosen to validate RNAseq results. QsAMS, QsLAP3, QsLAP5, QsLAP6 were selected as male candidate genes, whereas QsAt4g27290, QsCYP78A9, QsPG1, and QsSTIG1 as female candidate genes. Transcript abundance was determined using qPCR, and normalized to QsPP2AA3 using cDNA synthesized from distinct pools of RNA covering either early (1F and 1M) or late (2F and 2M) stages of male or female flower development. Reactions were performed in three biological and technical replicates. Error bars indicate standard deviation (SD).