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. 2014 Nov 6;5:599. doi: 10.3389/fpls.2014.00599

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Copyright © 2014 Rocheta, Sobral, Magalhães, Amorim, Ribeiro, Pinheiro, Egas, Morais-Cecílio and Costa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Description of the Quercus suber unique and differentially expressed genes. (A) Venn diagram indicating the number of exclusive and shared transcripts of early and late developmental stages of Quercus suber flower. Four EST projects were generated from four-specific RNA pools, two for female flowers (1F and 2F) and two for male flowers (1M and 2M), covering either early (1F and 1M) or the late (2F and 2M) developmental stages. The four individually EST projects were assembled into the 1F_1M_2F_2M library and the exclusive transcripts were identified using the Venny application (Oliveros, 2007). (B–J) Differentially expressed genes were clustered using the Self-organizing Trees algorithm (SOTA), euclidean distance (Dopazo and Carazo, 1997; Herrero et al., 2001) and the default settings of the MeV, MultiExperiment Viewer program (http://www.tm4.org/mev.html).