Table 1.

Sequencing and annotation statistics of Quercus suber flower libraries.

	1F	2F	1M	2M	1F_1M_2F_2M
Number of raw reads	332,607	312,282	255,962	270,871
Number of reads after trimming	280,092	252,024	205,781	224,275	837,163
Average read length after trimming	264	253	269	270	263
Number of contigs	7565	6923	5267	5171	16,832
Average contig length	773	714	779	812	914
Range of contig length	[60.. 3489]	[52.. 3394]	[36.. 3384]	[15.. 3392]	[16.. 3848]
Number of translated contigs	7289	6600	5090	4981	16,152
Peptide sequences with BLASTx matches	5723	5154	4083	3997	11,956
Peptide sequences translated by FrameDP	2211	1907	1386	1398	6621
Peptide sequences translated by ESTScan	108	117	116	114	297
Total of amino acid sequences	8042	7178	5585	5509	18,874
Peptide sequences with BLASTP matches	1294	1086	809	825	3372
Amino acid sequence assigned InterPro terms	5940	5312	4251	4217	12,698
Amino acid sequence assigned to GO terms	4536	4056	3272	3269	9459

Individual libraries were generated from four specific RNA pools, two for female flowers (1F and 2F) and two for male flowers (1M and 2M), covering either early (1F and 1M) or late (2F and 2M) stages of flower development. The four individually EST projects were assembled into the 1F_1M_2F_2M library. The libraries were assembled using 454 Newbler 2.6 (Roche) and annotated in a three-step process using BLASTx search, FrameDP and ESTScan using default parameters.