Table 2.
Candidate genes that were selected to validate the transcriptional levels determined by RNAseq results.
| Candidate genes | Gene accession | 1F | 2F | 1M | 2M |
|---|---|---|---|---|---|
| • At4g27290 | QSP078589.0 | 29 | 14 | 0 | 1 |
| • CYTOCHROME P450 78A9 | QSP091316.0 | 70 | 70 | 0 | 0 |
| • POLYGALACTURONASE1 | QS094531.0 | 19 | 337 | 0 | 0 |
| • STIGMA SPECIFIC1 | QS121989.0 | 5 | 34 | 0 | 0 |
| * ABORTED MICROSPORES | QS049646.0 | 0 | 0 | 38 | 4 |
| * LESS ADHERENT POLLEN3 | QS049611.0 | 1 | 2 | 170 | 46 |
| * LESS ADHESIVE POLLEN5 | QS003695.0 | 0 | 0 | 566 | 6 |
| * LESS ADHERENT POLLEN6 | QS039918.0 | 0 | 0 | 924 | 49 |
| PROTEIN PHOSPHATASE 2A SUBUNIT A3 | QS092015.0 | 55 | 71 | 57 | 65 |
The number of ESTs represents their distribution in the female and male Quercus suber combined flower library, covering either early (1F and 1M) or late (2F and 2M) stages of flower development. The selected genes were identified by homology with functional important genes known to be involved in carpel or stamen development.
•
Female candidate genes.
*
Male candidate genes.
PROTEIN PHOSPHATASE 2A SUBUNIT A3 was chosen as the reference gene. Gene accessions according to CorkOak database (www.corkoakdb.org).