Figure 2.

TVCV, but not CaMV, systemic spread is delayed in syta-1. (A) Coomassie blue-stained SDS-PAGE gels of systemic leaf extracts from four TVCV-inoculated Arabidopsis wt Col-0 or syta-1 (line 1) plants at 10 da post-inoculation. Positions of TVCV CP (17.5 kDa) and molecular weight markers (kDa) are indicated. (B) Semi-quantitative PCR analyses of CaMV genome DNA in systemic leaf extracts from mock inoculated control (C) or CaMV infected (inf) plants at 21 da post-inoculation. Shown are agarose gel analyses of extracts from Arabidopsis wt Col-0 plants and the two independent mutant lines syta-1 (line 1) (syta-1¹) and syta-1 (line 16) (syta-1¹⁶). Positions of PCR products of CaMV CP gene and SYTB gene (internal loading control), and of molecular weight markers (nt) are indicated. Equal amounts of systemic leaf extracts were analyzed in each gel in panels (A) and (B).