Table 3.
MP cell-to-cell trafficking in syta-1 and wild type Col-0.
| MP | Trial | Line | Total foci (cells) | 1 cells (%)a | ≥2cells (%)a | P(χ2)b |
|---|---|---|---|---|---|---|
| GFP-MPCaLCuVc | 1 | wt | 45 (165) | 28.9 | 71.1 | <0.0001 |
| syta-1 | 46 (136) | 50.0 | 50.0 | |||
| 2 | wt | 33 (158) | 21.2 | 78.8 | ||
| syta-1 | 32 (94) | 46.9 | 53.1 | |||
| 3 | wt | 33 (150) | 12.1 | 87.8 | ||
| syta-1 | 30 (74) | 56.7 | 43.3 | |||
| MPTVCV-GFPc | 1 | wt | 25 (124) | 8.0 | 92.0 | <0.0001 |
| syta-1 | 30 (97) | 30.0 | 70.0 | |||
| 2 | wt | 15 (92) | 6.7 | 93.3 | ||
| syta-1 | 25 (68) | 58.8 | 41.2 | |||
| 3 | wt | 17 (77) | 13.8 | 86.2 | ||
| syta-1 | 20 (64) | 30.0 | 70.0 | |||
| 4 | wt | 29 (117) | 13.8 | 86.2 | ||
| syta-1 | 26 (69) | 34.6 | 65.4 | |||
| P3N-PIPOTuMV-GFPc | 1 | wt | 21 (44) | 38.1 | 61.9 | <0.0001 |
| syta-1 | 17 (23) | 64.7 | 35.3 | |||
| 2 | wt | 26 (48) | 42.3 | 57.7 | ||
| syta-1 | 28 (35) | 75.0 | 25.0 | |||
| 3 | wt | 27 (58) | 40.7 | 59.3 | ||
| syta-1 | 29 (33) | 89.7 | 10.3 | |||
| 4 | wt | 27 (47) | 48.1 | 51.9 | ||
| syta-1 | 26 (30) | 88.5 | 11.5 | |||
| t-TM-GFPd | 1 | wt | 57 (60) | 94.7 | 5.3d | ---- |
| 2 | wt | 58 (60) | 96.6 | 3.4d | ||
| 3 | wt | 40 (41) | 97.5 | 2.5d | ||
| 4 | wt | 40 (40) | 100.0 | 0.0d |
a
Percent fluorescent cells where each MP was in a single cell (1 cell), or moved to 2 or more cells (≥2).
b
χ2 test for cell-to-cell movement of each MP bombarded into syta-1 vs. wild type Col-0 leaves for all three or four trails shown.
c
Each MP was assayed for cell-to-cell movement at the following times post bombardment: MPCaLCuV 36–46 h, MPTVCV 24 h, P3N-PIPOTuMV 48 h, MPCaMV 24 h.
d
Golgi marker: transmembrane domain (TM) of rat sialotranferase fused to GFP. No difference was found in the percentage of foci with 1 vs. 2 labeled cells for syta-1 vs. wild type Col-0. Foci with >2 adjacent t-TM-GFP-labeled cells were never observed. Foci were imaged at 12–24 h post bombardment.