Table 2.
Impact of catecholamines on the expression of flagellar motility-related genes in V. harveyi.
| Gene | Function | Relative expression (fold)1 |
||
|---|---|---|---|---|
| Untreated | Norepinephrine (NE) (50 μM) | Dopamine (Dopa) (50 μM) | ||
| flaA | Polar flagellin | 1.0 ± 0.1 | 2.8 ± 0.4** | 1.6 ± 0.1** |
| flaC | Polar flagellin | 1.0 ± 0.1 | 1.9 ± 0.1*** | 1.3 ± 0.2* |
| flaK | Polar flagellar regulator | 1.0 ± 0.3 | 2.9 ± 0.2*** | 1.7 ± 0.3** |
| fliA | Polar flagellar biosynthesis sigma factor | 1.0 ± 0.4 | 2.9 ± 0.5** | 2.2 ± 0.1*** |
| fliS | Polar flagellin specific chaperone | 1.0 ± 0.3 | 3.2 ± 0.5** | 1.7 ± 0.1*** |
| flgB | Flagellar basal body rod | 1.0 ± 0.2 | 3.2 ± 0.3** | 1.4 ± 0.4** |
| cheA | Chemotaxis protein | 1.0 ± 0.1 | 2.5 ± 0.3*** | 1.9 ± 0.2** |
| cheR | Chemotaxis protein | 1.0 ± 0.4 | 2.0 ± 0.1** | 1.4 ± 0.3* |
| lafA | Lateral flagellar flagellin | 1.0 ± 0.3 | 3.0 ± 0.4** | 1.9 ± 0.1*** |
| lafK | Lateral flagellar regulator | 1.0 ± 0.2 | 3.0 ± 0.6** | 1.6 ± 0.4** |
1For each gene, the expression in untreated cultures was set at 1 and the expression in cultures supplemented with catecholamines was normalized accordingly using the 2-ΔΔCt method. Data are average ± SD of three V. harveyi cultures. Asterisks indicate a significant difference when compared to untreated V. harveyi (independent samples t-test; *p < 0.05; **p < 0.01; ***p < 0.001).