. 2014 Nov 6;5:584. doi: 10.3389/fmicb.2014.00584

Table 3.

Impact of pretreatment of V. harveyi with catecholamines and catecholamine receptor antagonists on virulence of the bacterium toward gnotobiotic brine shrimp larvae.

Treatment	Survival (%)^a
Control	100 ± 0^c
V. harveyi	50 ± 5^b
V. harveyi [50 μM NE]	27 ± 6^a
V. harveyi [50 μM NE + 100 μM phentolamine]	48 ± 3^b
V. harveyi [50 μM NE + 10 μM phenoxybenzamine]	47 ± 3^b
V. harveyi [50 μM NE + 50 μM labetalol]	48 ± 3^b
V. harveyi [50 μM NE + 100 μM propanolol]	29 ± 4^a
V. harveyi [50 μM NE + 5 pM LED209]	55 ± 5^b
V. harveyi [50 μM Dopa]	35 ± 5^a
V. harveyi [50 μM Dopa + 50 μM chlorpromazine]	47 ± 3^b
V. harveyi [50 μM Dopa + 5 pM LED209]	52 ± 8^b

^aSurvival after 2 days of challenge with V. harveyi ATCC BAA-1116 (average ± SE of four replicates). Survival in the control treatment was set at 100% and the other treatments were normalized accordingly. Square brackets refer to pretreatment – V. harveyi was either or not pretreated with catecholamines and antagonists for 4 h and washed prior to inoculation into the brine shrimp rearing water. Values with a different superscript letter are significantly different from each other (One way ANOVA with Tukey’s post hoc test; p < 0.01).