Figure 2.

The burden of minor histoincompatibility in human SCT. (A) All possible mHA in human beings: data generated from NCBI dbSNP database (22). (B) Alloreactivity potential: the current patient cohort had an average of 6,445 nsSNPs/DRP, which when converted into peptide fragments averaged 486,463 possible mHA/DRP. (C) Putative mHA: each DRP had its nsSNP_GVH-encoded peptides filtered by predicted binding to six HLA class I alleles specific to that DRP. Average number of peptides with binding affinity labeled presented (<500 nM), and strongly presented (<50 nM) is shown.