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. 2014 Nov 6;5:607. doi: 10.3389/fpls.2014.00607

Table 1.

Validation of microarray data.

Gene FC Cig_4Ba FC Cig_B510 FC Nip_4B FC Nip_B510b
Log2 (qPCR) Log2 (array) Log2 (qPCR) Log2 (array) Log2 (qPCR) Log2 (array) Log2 (qPCR) Log2 (array)
Differentially expressed in all conditions
Os09g0358000 Tyrosine kinase domain 2.27 1.77 2.53 2.46 1.87 3.01 2.06 1.32
Differentially expressed in Cig_4B and Nip_4B only
Os09g0417800 WRKY transcription factor 62 1.49 1.75 0.66 _ 1.00 1.88 −0.01 _
Os11g0686900 Similar to NB-ARC domain 0.83 1.16 −0.23 _ 1.74 2.40 nd _
Differentially expressed in Cig_B510 and Nip_B510 only
Os06g0115600 Common symbiosis signaling (SYM) pathway −0.09 _ −2.00 −1.84 0.01 _ −1.18 −1.47
Os05g0583000 Similar to WRKY8 0.32 _ 2.92 1.71 0.16 _ 2.24 1.39
Os12g0139400 A-type response regulator, cytokinin signaling −0.15 _ −2.84 −1.78 −0.09 _ −2.94 −1.10
Os11g0143300 A-type response regulator, cytokinin signaling 0.15 _ −1.09 −1.95 0.04 _ −2.25 −1.62
Os02g0805100 Similar to auxin-responsive protein IAA12 −0.01 _ −1.15 −1.34 −0.23 _ −2.47 −1.16
Differentially expressed in Cig_4B and Cig_B510 only
Os05g0196600 Similar to ACC synthase 0.38 1.33 0.95 1.63 −0.15 _ 0.16 _
Differentially expressed in Cig_4B only
Os08g0136100 Homeobox-leucine zipper domain 0.87 1.11 0.25 _ 0.28 _ −0.14 _
Significantly regulated in Cig_B510 only
Os06g0179200 Similar to Nodulin-like protein 0.16 _ −1.60 −1.78 0.18 _ 0.12 _
Os08g0499300 WRKY transcription factor 30 0.10 _ 0.52 1.34 0.12 _ −0.06 _
Significantly regulated in Nip_B510 only
Os01g0904700 B-type response regulator, cytokinin signaling −0.12 _ −0.07 _ −0.04 _ −1.09 −1.21
Os05g0515400 Similar to auxin response factor 14 −0.09 _ 0.10 _ −0.09 _ −1.15 −1.42
Reference gene
Os03g0718100 Actin
a

Dashes replace non-significant fold change values obtained from microarray data (|Log2(FC)| ≤ 1 and Padj > 0.05).

b

nd, not determined.