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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Protein Expr Purif. 2013 Dec 28;95:149–155. doi: 10.1016/j.pep.2013.12.010

Figure 5. The purified R318-His6 is functional in mediating PA63 pore formation at low pH.

Figure 5

The receptor-mediated PA63 pore formation was tested on the liposomal membranes using time-lapse intensity measurement of ANTS/DPX dequenching as described in Material and Methods. PA alone, buffer without proteins, and Triton X-100 (final concentration 0.06%) were used as negative or positive controls, respectively. The representative recordings of ANTS dequenching are shown in A. The fluorescence intensity at 60 seconds of post-acidification was quantified from two independent measurements and shown in B. Note: the fluorescence intensity induced by Triton X-100 was displayed in the y-axis on the right side of the graph.