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α-SMA, VIM, FN and MMP9 expression in WT and AKT-silenced cells treated with EVE. Histograms represent A) α-SMA, B) VIM, C) FN and D) MMP9 mRNA expression levels determined by real-time PCR. WT and AKT-silenced cells were cultured with Everolimus (10 and 100 nM) for 6 h. Mean ± S.D. (error bars) of three separate experiments performed in triplicate. **p < 0.001 versus WT CTR.
