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Mesenchymal Sox9 deletion did not alter lung branching. (A, C) Left lobes of (A) wild-type and (B) mutant lung at embryonic day (E) 15.5. Terminal branches were counted, and no difference in branching morphogenesis was identified. (D-G) Immunostaining for Sftpc, T1-αc, and Pecam in E18.5 lungs confirmed that distal lung epithelium and mesenchyme differentiation were not altered by Sox9 deletion in the lung mesenchyme.
