Figure 1.

Endothelial loss of CCM1 results in abnormal embryonic vascular development. (A and B) Visualization of the vasculature, as indicated by lectin stain, or whole-mount embryos at E9.5. (C and E) Close-up image of the branchial arch artery morphology from the indicated white outlines in A and B. (D and F) Illustration of the branchial arch artery from the adjacent panel, included to highlight visualization. (G and H) Brightfield images of ink injected E9.5 embryos. Light blue arrows indicate areas where ink was able to flow through the vasculature. (I and J) In utero ultrasound of blood flow on E9.5 embryos. Embryos are located in the center of each image with the box indicating the area being monitored for flow. The heat map bar on the left indicates the directionality of the blood flow, red indicates blood flow towards the camera, blue indicates blood flow away from the camera. Absence of color demonstrates absence of flow.