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. 2014 Jul 7;23(23):6260–6274. doi: 10.1093/hmg/ddu345

Figure 1.

Figure 1.

Genetic knockin of the Y141C mutation in RDS does not have an impact on RDS expression levels. The Y141C mutation was introduced into exon 1 of the RDS gene in order to drive properly regulated expression of the Y141C-RDS allele (A). Total RNA was isolated from the retinas of mice of the indicated genotypes at P30 and subjected to qRT-PCR for RDS and the housekeeping gene HPRT (B). Total RNA was also subjected to northern blotting using radiolabeled RDS cDNA as a probe (C). 28S and 18S ribosomal RNA are marked for size reference and 28S is shown as loading control. Levels of RDS protein in total retinal extracts were analyzed from indicated genotypes by WB followed by visualization using antibodies specific to RDS, ROM-1 and actin (D). Band densities were normalized to actin and set relative to WT: RDS (E), ROM-1 (F). Shown are mean ± SEM. N = 3 (B and C) and 8 (E and F) independent samples. ***P < 0.001 by one-way ANOVA with Bonferroni's post hoc comparison.